Part:BBa_K5091011

Native degQ Expression Construct

This composite part is designed to express the degQ gene using its native promoter and terminator sequences. The construct consists of the following components:

PdegQ Promoter (BBa_K3429006): This is the native promoter for the degQ gene, allowing for natural regulation of degQ expression. The use of a native promoter ensures that the gene is expressed at levels similar to those found in its original organism, making it ideal for studies that aim to maintain physiological expression patterns.

RBS (BBa_B0034): A standard ribosome binding site that ensures efficient translation initiation, facilitating proper protein synthesis in the host organism.

degQ Sequence (BBa_K5091005): This is the coding sequence for the degQ gene from Bacillus subtilis, which enhances fengycin production, a key antifungal compound used in biocontrol of fungal pathogens. The degQ protein regulates fengycin biosynthesis, making this part useful for agricultural or biocontrol projects aimed at improving plant resistance to pathogens.

DegQ Terminator (BBa_K3429007): This is the native terminator for the degQ gene, ensuring proper transcription termination and mRNA stability, which is essential for the efficient production of the DegQ protein.

This construct is ideal for projects that require natural expression of the degQ gene, particularly in systems where maintaining native regulation is important for studying the physiological role of DegQ in fengycin production. The part can be used in microbial systems to boost biocontrol capabilities against plant pathogens.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]